IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling
Identifieur interne : 004649 ( Main/Exploration ); précédent : 004648; suivant : 004650IMGT/HighV QUEST paradigm for T cell receptor IMGT clonotype diversity and next generation repertoire immunoprofiling
Auteurs : Shuo Li [Australie] ; Marie-Paule Lefranc [France] ; John J. Miles [Australie, Royaume-Uni] ; Eltaf Alamyar [France] ; Véronique Giudicelli [France] ; Patrice Duroux [France] ; J. Douglas Freeman [Canada] ; Vincent D. A. Corbin [Australie] ; Jean-Pierre Scheerlinck [Australie] ; Michael A. Frohman [États-Unis] ; Paul U. Cameron [Australie] ; Magdalena Plebanski [Australie] ; Bruce Loveland [Australie] ; Scott R. Burrows [Australie] ; Anthony T. Papenfuss [Australie] ; Eric J. Gowans [Australie]Source :
- Nature Communications [ 2041-1723 ] ; 2013.
Abstract
T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5′RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB.
Url:
DOI: 10.1038/ncomms3333
PubMed: 23995877
PubMed Central: 3778833
Affiliations:
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Miles</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Queensland Institute of Medical Research</institution>, Brisbane, Queensland 4006,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a6"><institution>Institute of Infection and Immunity, Cardiff University</institution>, Cardiff CF14 4XN,<country>UK</country></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a7"><institution>School of Medicine, University of Queensland</institution>, Brisbane, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff id="a15">These authors contributed equally to this work</nlm:aff></affiliation></author><author><name sortKey="Alamyar, Eltaf" sort="Alamyar, Eltaf" uniqKey="Alamyar E" first="Eltaf" last="Alamyar">Eltaf Alamyar</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>IMGT, the international ImMunoGeneTics information system, Université Montpellier 2, Institut de Génétique Humaine, UPR CNRS, 1142</institution> Montpellier 34396,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Giudicelli, Veronique" sort="Giudicelli, Veronique" uniqKey="Giudicelli V" first="Véronique" last="Giudicelli">Véronique Giudicelli</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>IMGT, the international ImMunoGeneTics information system, Université Montpellier 2, Institut de Génétique Humaine, UPR CNRS, 1142</institution> Montpellier 34396,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Duroux, Patrice" sort="Duroux, Patrice" uniqKey="Duroux P" first="Patrice" last="Duroux">Patrice Duroux</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>IMGT, the international ImMunoGeneTics information system, Université Montpellier 2, Institut de Génétique Humaine, UPR CNRS, 1142</institution> Montpellier 34396,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Freeman, J Douglas" sort="Freeman, J Douglas" uniqKey="Freeman J" first="J. Douglas" last="Freeman">J. Douglas Freeman</name><affiliation wicri:level="1"><nlm:aff id="a8"><institution>Michael Smith Genome Sciences Centre</institution>, Vancouver, British Columbia,<country>Canada</country> V5Z 1L3</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Corbin, Vincent D A" sort="Corbin, Vincent D A" uniqKey="Corbin V" first="Vincent D. A." last="Corbin">Vincent D. A. Corbin</name><affiliation wicri:level="1"><nlm:aff id="a9"><institution>Bioinformatics division, The Walter & Eliza Hall Institute of Medical Research</institution>, Parkville, Victoria 3052,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a10"><institution>Department of Medical Biology, University of Melbourne</institution>, Melbourne, Victoria 3010,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Scheerlinck, Jean Pierre" sort="Scheerlinck, Jean Pierre" uniqKey="Scheerlinck J" first="Jean-Pierre" last="Scheerlinck">Jean-Pierre Scheerlinck</name><affiliation wicri:level="1"><nlm:aff id="a11"><institution>Centre for Animal Biotechnology, University of Melbourne</institution>, Melbourne, Victoria 3010,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Frohman, Michael A" sort="Frohman, Michael A" uniqKey="Frohman M" first="Michael A." last="Frohman">Michael A. Frohman</name><affiliation wicri:level="1"><nlm:aff id="a12"><institution>The Department of Pharmacology and the Centre for Developmental Genetics, Stony Brook University</institution>, Stony Brook, New York 11794,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Cameron, Paul U" sort="Cameron, Paul U" uniqKey="Cameron P" first="Paul U." last="Cameron">Paul U. Cameron</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Burnet Institute</institution>, Melbourne, Victoria 3004,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Plebanski, Magdalena" sort="Plebanski, Magdalena" uniqKey="Plebanski M" first="Magdalena" last="Plebanski">Magdalena Plebanski</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Department of Immunology, Monash University</institution>, Melbourne, Victoria 3004,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Loveland, Bruce" sort="Loveland, Bruce" uniqKey="Loveland B" first="Bruce" last="Loveland">Bruce Loveland</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Burnet Institute</institution>, Melbourne, Victoria 3004,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Department of Immunology, Monash University</institution>, Melbourne, Victoria 3004,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Burrows, Scott R" sort="Burrows, Scott R" uniqKey="Burrows S" first="Scott R." last="Burrows">Scott R. Burrows</name><affiliation wicri:level="1"><nlm:aff id="a5"><institution>Queensland Institute of Medical Research</institution>, Brisbane, Queensland 4006,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Papenfuss, Anthony T" sort="Papenfuss, Anthony T" uniqKey="Papenfuss A" first="Anthony T." last="Papenfuss">Anthony T. Papenfuss</name><affiliation wicri:level="1"><nlm:aff id="a9"><institution>Bioinformatics division, The Walter & Eliza Hall Institute of Medical Research</institution>, Parkville, Victoria 3052,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a10"><institution>Department of Medical Biology, University of Melbourne</institution>, Melbourne, Victoria 3010,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a13"><institution>Department of Mathematics and Statistics, University of Melbourne</institution>, Victoria 3010,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Gowans, Eric J" sort="Gowans, Eric J" uniqKey="Gowans E" first="Eric J." last="Gowans">Eric J. Gowans</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Burnet Institute</institution>, Melbourne, Victoria 3004,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a14"><institution>Discipline of Surgery, University of Adelaide</institution>, Adelaide, South Australia 5011,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Nature Communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>T cell repertoire diversity and clonotype follow-up in vaccination, cancer, infectious and immune diseases represent a major challenge owing to the enormous complexity of the data generated. Here we describe a next generation methodology, which combines 5′RACE PCR, 454 sequencing and, for analysis, IMGT, the international ImMunoGeneTics information system (IMGT), IMGT/HighV-QUEST web portal and IMGT-ONTOLOGY concepts. The approach is validated in a human case study of T cell receptor beta (TRB) repertoire, by chronologically tracking the effects of influenza vaccination on conventional and regulatory T cell subpopulations. The IMGT/HighV-QUEST paradigm defines standards for genotype/haplotype analysis and characterization of IMGT clonotypes for clonal diversity and expression and achieves a degree of resolution for next generation sequencing verifiable by the user at the sequence level, while providing a normalized reference immunoprofile for human TRB.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author><author><name sortKey="Lefranc, G" uniqKey="Lefranc G">G. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Arstila, T P" uniqKey="Arstila T">T. P. Arstila</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nikolich Zugich, J" uniqKey="Nikolich Zugich J">J. Nikolich-Zugich</name></author><author><name sortKey="Slifka, M K" uniqKey="Slifka M">M. K. Slifka</name></author><author><name sortKey="Messaoudi, I" uniqKey="Messaoudi I">I. Messaoudi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rudolph, M G" uniqKey="Rudolph M">M. G. Rudolph</name></author><author><name sortKey="Stanfield, R L" uniqKey="Stanfield R">R. L. Stanfield</name></author><author><name sortKey="Wilson, I A" uniqKey="Wilson I">I. A. Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaas, Q" uniqKey="Kaas Q">Q. Kaas</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Douek, D C" uniqKey="Douek D">D. C. Douek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miles, J J" uniqKey="Miles J">J. J. Miles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, D A" uniqKey="Price D">D. A. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, D A" uniqKey="Price D">D. A. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kedzierska, K" uniqKey="Kedzierska K">K. Kedzierska</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miles, J J" uniqKey="Miles J">J. J. Miles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Freeman, J D" uniqKey="Freeman J">J. D. Freeman</name></author><author><name sortKey="Warren, R L" uniqKey="Warren R">R. L. Warren</name></author><author><name sortKey="Webb, J R" uniqKey="Webb J">J. R. Webb</name></author><author><name sortKey="Nelson, B H" uniqKey="Nelson B">B. H. Nelson</name></author><author><name sortKey="Holt, R A" uniqKey="Holt R">R. A. Holt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Warren, R L" uniqKey="Warren R">R. L. Warren</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robins, H S" uniqKey="Robins H">H. S. Robins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Robins, H S" uniqKey="Robins H">H. S. Robins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Venturi, V" uniqKey="Venturi V">V. Venturi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nguyen, P" uniqKey="Nguyen P">P. Nguyen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Klarenbeek, P L" uniqKey="Klarenbeek P">P. L. Klarenbeek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fohse, L" uniqKey="Fohse L">L. Fohse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alamyar, E" uniqKey="Alamyar E">E. Alamyar</name></author><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author><author><name sortKey="Duroux, P" uniqKey="Duroux P">P. Duroux</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alamyar, E" uniqKey="Alamyar E">E. Alamyar</name></author><author><name sortKey="Duroux, P" uniqKey="Duroux P">P. Duroux</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brochet, X" uniqKey="Brochet X">X. Brochet</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Brochet, X" uniqKey="Brochet X">X. Brochet</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frohman, M A" uniqKey="Frohman M">M. A. Frohman</name></author><author><name sortKey="Dush, M K" uniqKey="Dush M">M. K. Dush</name></author><author><name sortKey="Martin, G R" uniqKey="Martin G">G. R. Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seddiki, N" uniqKey="Seddiki N">N. Seddiki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fontenot, J D" uniqKey="Fontenot J">J. D. Fontenot</name></author><author><name sortKey="Rudensky, A Y" uniqKey="Rudensky A">A. Y. Rudensky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, S" uniqKey="Li S">S. Li</name></author><author><name sortKey="Gowans, E J" uniqKey="Gowans E">E. J. Gowans</name></author><author><name sortKey="Chougnet, C" uniqKey="Chougnet C">C. Chougnet</name></author><author><name sortKey="Plebanski, M" uniqKey="Plebanski M">M. Plebanski</name></author><author><name sortKey="Dittmer, U" uniqKey="Dittmer U">U. Dittmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Chaume, D" uniqKey="Chaume D">D. Chaume</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. 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Giudicelli</name></author><author><name sortKey="Chaume, D" uniqKey="Chaume D">D. Chaume</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bleakley, K" uniqKey="Bleakley K">K. Bleakley</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author><author><name sortKey="Biau, G" uniqKey="Biau G">G. Biau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Giudicelli, V" uniqKey="Giudicelli V">V. Giudicelli</name></author><author><name sortKey="Lefranc, M P" uniqKey="Lefranc M">M.-P. Lefranc</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nguyen, P" uniqKey="Nguyen P">P. Nguyen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miles, J J" uniqKey="Miles J">J. J. Miles</name></author><author><name sortKey="Douek, D C" uniqKey="Douek D">D. C. Douek</name></author><author><name sortKey="Price, D A" uniqKey="Price D">D. A. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miles, J J" uniqKey="Miles J">J. J. Miles</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, D A" uniqKey="Price D">D. A. Price</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menezes, J S" uniqKey="Menezes J">J. S. Menezes</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li><li>Canada</li><li>France</li><li>Royaume-Uni</li><li>États-Unis</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Li, Shuo" sort="Li, Shuo" uniqKey="Li S" first="Shuo" last="Li">Shuo Li</name></noRegion><name sortKey="Burrows, Scott R" sort="Burrows, Scott R" uniqKey="Burrows S" first="Scott R." last="Burrows">Scott R. Burrows</name><name sortKey="Cameron, Paul U" sort="Cameron, Paul U" uniqKey="Cameron P" first="Paul U." last="Cameron">Paul U. Cameron</name><name sortKey="Corbin, Vincent D A" sort="Corbin, Vincent D A" uniqKey="Corbin V" first="Vincent D. A." last="Corbin">Vincent D. A. Corbin</name><name sortKey="Corbin, Vincent D A" sort="Corbin, Vincent D A" uniqKey="Corbin V" first="Vincent D. A." last="Corbin">Vincent D. A. Corbin</name><name sortKey="Gowans, Eric J" sort="Gowans, Eric J" uniqKey="Gowans E" first="Eric J." last="Gowans">Eric J. Gowans</name><name sortKey="Gowans, Eric J" sort="Gowans, Eric J" uniqKey="Gowans E" first="Eric J." last="Gowans">Eric J. Gowans</name><name sortKey="Li, Shuo" sort="Li, Shuo" uniqKey="Li S" first="Shuo" last="Li">Shuo Li</name><name sortKey="Li, Shuo" sort="Li, Shuo" uniqKey="Li S" first="Shuo" last="Li">Shuo Li</name><name sortKey="Loveland, Bruce" sort="Loveland, Bruce" uniqKey="Loveland B" first="Bruce" last="Loveland">Bruce Loveland</name><name sortKey="Loveland, Bruce" sort="Loveland, Bruce" uniqKey="Loveland B" first="Bruce" last="Loveland">Bruce Loveland</name><name sortKey="Miles, John J" sort="Miles, John J" uniqKey="Miles J" first="John J." last="Miles">John J. Miles</name><name sortKey="Miles, John J" sort="Miles, John J" uniqKey="Miles J" first="John J." last="Miles">John J. Miles</name><name sortKey="Papenfuss, Anthony T" sort="Papenfuss, Anthony T" uniqKey="Papenfuss A" first="Anthony T." last="Papenfuss">Anthony T. Papenfuss</name><name sortKey="Papenfuss, Anthony T" sort="Papenfuss, Anthony T" uniqKey="Papenfuss A" first="Anthony T." last="Papenfuss">Anthony T. Papenfuss</name><name sortKey="Papenfuss, Anthony T" sort="Papenfuss, Anthony T" uniqKey="Papenfuss A" first="Anthony T." last="Papenfuss">Anthony T. Papenfuss</name><name sortKey="Plebanski, Magdalena" sort="Plebanski, Magdalena" uniqKey="Plebanski M" first="Magdalena" last="Plebanski">Magdalena Plebanski</name><name sortKey="Scheerlinck, Jean Pierre" sort="Scheerlinck, Jean Pierre" uniqKey="Scheerlinck J" first="Jean-Pierre" last="Scheerlinck">Jean-Pierre Scheerlinck</name></country><country name="France"><noRegion><name sortKey="Lefranc, Marie Paule" sort="Lefranc, Marie Paule" uniqKey="Lefranc M" first="Marie-Paule" last="Lefranc">Marie-Paule Lefranc</name></noRegion><name sortKey="Alamyar, Eltaf" sort="Alamyar, Eltaf" uniqKey="Alamyar E" first="Eltaf" last="Alamyar">Eltaf Alamyar</name><name sortKey="Duroux, Patrice" sort="Duroux, Patrice" uniqKey="Duroux P" first="Patrice" last="Duroux">Patrice Duroux</name><name sortKey="Giudicelli, Veronique" sort="Giudicelli, Veronique" uniqKey="Giudicelli V" first="Véronique" last="Giudicelli">Véronique Giudicelli</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Miles, John J" sort="Miles, John J" uniqKey="Miles J" first="John J." last="Miles">John J. Miles</name></noRegion></country><country name="Canada"><noRegion><name sortKey="Freeman, J Douglas" sort="Freeman, J Douglas" uniqKey="Freeman J" first="J. Douglas" last="Freeman">J. Douglas Freeman</name></noRegion></country><country name="États-Unis"><noRegion><name sortKey="Frohman, Michael A" sort="Frohman, Michael A" uniqKey="Frohman M" first="Michael A." last="Frohman">Michael A. Frohman</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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